廖春雨博士，深圳理工大学（筹）生命健康学院科研正教授，博士生导师，国家级青年人才。2015年在美国爱荷华州立大学获博士学位。2016-2021年，在美国北卡罗莱纳州立大学和德国Helmholtz Institute of RNA Based Infection Research从事科学研究。廖春雨博士长期专注于细菌防御系统的生物学与分子机理研究，以及基因编辑工具的开发、优化、应用。近年来，研究成果发表于Nature Microbiology、Nature Communications、Annual Review of Genetics、Molecular Cell、Nucleic Acid Research、PNAS等期刊。开发的CRISPR阵列的构建方法得到广泛应用，关于CRISPR RNA biogenesis的系统性研究在领域内得到广泛认可。
学习经历：
2010—2015爱荷华州立大学（Iowa State University），微生物学，博士
2007—2010华中农业大学，微生物学，硕士
2003—2007湖北工业大学，生物工程，学士
工作经历：
2024至今深圳理工大学（筹），生命健康学院生物学系历任助理教授、科研正教授
2018—2021亥姆霍兹研究所传染病分所（Helmholtz Institute of RNA Based Infection Research），助理研究员
2016—2018北卡莱罗那州立大学（North Carolina State University），博士后
·国际影响力
Oralpresentation,Modular, one-pot assembly of CRISPR arrays reveals factors influencing
crRNA biogenesis, Genome Editing with CRISPR Conference,德国柏林, 2019-9-4至2019-9-6
Oralpresentation,One-Step Assembly of Class 2 CRISPR Arrays for Multiplexed Cleavage and
Regulation, International Conference on CRISPR Technologies,美国北卡莱罗那州罗利市, 2017-12-4至2017-12-6
Paper of the Month Award,亥姆霍兹传染病研究院(HZI), 2019
·科研成果
近年来，研究成果发表于Nature Microbiology、Nature Communications、Annual Review of Genetics、Molecular Cell、Nucleic Acid Research、PNAS等期刊。开发的CRISPR阵列的构建方法得到广泛应用，关于CRISPR RNA biogenesis的系统性研究在领域内得到广泛认可。
·代表性论文：
1. Liao C, Sharma S, Svensson SL, Kibe A, Weinberg Z, Alkhnbashi SO, Bischler T, Backofen R, Caliskan N, Sharma CM, Beisel CL. (2022) Spacer prioritization in CRISPR-Cas9 immunity is enabled by the leader RNA. Nature Microbiology. doi: 10. 1038/s41564-022-01074-3
2. Liao C, Beisel CL. (2021) The tracrRNA in CRISPR Biology and Technologies. Annual review of genetics. doi: 10. 1146/annurev-genet-071719-022559.
3. Liao C, Ttofali F, Slotkowski RA, Denny SR, Cecil, TD, Leenay, RT, Keung, AJ, Beisel, CL.
(2019) Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis. Nature Communications. doi: 10. 1038/s41467-019-10747-3.
4. Liao C, Slotkowski RA, Achmedov T, Beisel CL. (2019) The Francisella novicida Cas12a is sensitive to the structure downstream of the terminal repeat in CRISPR arrays. RNA Biology. doi: 10. 1080/15476286.2018.1526537.
5. Wu WY, Mohanraju P, Liao C, Adiego-Pérez B, Creutzburg SCA, Makarova KS, Keessen K, Khan TS, Prinsen S, Yan WX, Migur A, Laffeber C, Scott DA, Lebbink JHG, Koonin EV, Beisel CL, Oost JVD. (2022) The miniature CRISPR-Cas12m effector binds DNA to block transcription. Molecular Cell. doi: 10. 1016/j.molcel.2022. 11.003
6. Liao C, Slotkowski RA, Beisel CL. (2019) CRATES: A one-step assembly method for Class 2 CRISPR arrays. Methods in Enzymology. doi: 10. 1016/bs.mie.2019.04.011.
7. Prezza G, Liao C, Reichardt S, Beisel CL, Westermann AJ. (2024) CRISPR-based screening of small RNA modulators of bile susceptibility in Bacteroides thetaiotaomicron. PNAS. doi: 10. 1073/pnas.2311323121.
8. Jacobsen T, Ttofali F, Liao C, Manchalu S, Gray BN, Beisel CL. (2020) Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs. Nucleic Acids Research. doi: 10. 1093/nar/gkaa272.
9. Collins SP, Rostain W, Liao C, Beisel CL. (2021) Sequence-independent RNA sensing and DNA targeting by a split domain CRISPR-Cas12a gRNA switch. Nucleic Acids Research. doi: 10. 1093/nar/gkab100.
10. Jacobsen T, Liao C, Beisel CL. (2019) The Acidaminococcus sp. Cas12a nuclease recognizes GTTV and GCTV as non-canonical PAMs. FEMS Microbiology Letters. doi: 10. 1093/femsle/fnz085.
11. Gawlitt S, Liao C, Achmedov T, Beisel CL. (2023) Shortened CRISPR-Cas9 arrays enable multiplexed gene targeting in bacteria from a smaller DNA footprint. RNA Biology. doi: 10. 1080/15476286.2023.2247247.
